Cell Lines: Cell Culture, Hela, Hek Cell, Caco-2, Sh-Sy5y, Mcf-7, Vero Cell, K562 Cells, Hep G2, Hl60, A431 Cells, U937 Cells

Purchase includes free access to book updates online and a free trial membership in the publisher's book club where you can select from more than a million books without charge. Chapters: Cell Culture, Hela, Hek Cell, Caco-2, Sh-Sy5y, Mcf-7, Vero Cell, K562 Cells, Hep G2, Hl60, A431 Cells, U937 Cells, Chinese Hamster Ovary Cell, H1299, Jurkat Cells, Lncap, 3t3 Cells, Pc12 Cells, Thp1 Cell Line, Vg-1, Du145, Pc3, Sf21, Cos Cells, 3t3-L1, C2c12, Ab9, A549 Cell, Jy Cell Line. Excerpt: 3T3-L1 is a cell line derived from 3T3 cells that is used in biological research on adipose tissue . 3T3-L1 cells have a fibroblast -like morphology , but, under appropriate conditions, the cells differentiate into an adipocyte -like phenotype . 3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. These cells are also sensitive to lipogenic and lipolytic hormones and drugs, including epinephrine , isoproterenol , and insulin . Sources A hyperlinked version of this chapter is at NIH/3T3 Fibroblasts in cell culture 3T3 cells come from a cell line established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. The 3T3 cell line has become the standard fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from Swiss mouse embryo tissue. Nomenclature The '3T3' designation refers to the abbreviation of "3-day transfer, inoculum 3 x 10 cells." This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 x 10 cells per 20-cm² dish (the second "3") continuously. The spontaneously immortalized cells with stable growth rate were established afte...