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Andhra Pradesh Board Biotechnology Sample Paper
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Andhra Pradesh Board Biotechnology Sample Paper
UNIT-I
INTRODUCTION TO BIOTECHNOLOGY:-
Fundamentals of biochemical engineering Biotechnology and society.
UNIT-II
BIOMOLECULES:-
Building blocks of Biomolecules and dynamic structure and
functions of macromolecules, Biochemical Techniques.fs
UNIT-III
CELL AND DEVELOPMENT:-
The basic unit of life.
Cell growth and development
Cellular Techniques.
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UNIT-IV
GENITECS AND MOLECULAR BIOLOGY:-
Principles of genetics
Geneme functions
Genetical Techniques.
PRACTICALS
Note:- Every student is required to do the following Experiments during the acadamic
session.
List of Experiments:-
1. Preparation of buffers and PH determination.
2. Sterlization techniques (wet and dry sterlization, chemical sterlization and ultra filtration.)
3. Media preparation (Solid and liquid LB Medium)
4. Isolation of Bacteria from curd and staining of Bacteria.
5. Determination of Bacterial growth curve.
6. Study of various stages of mitosis and calculation of mitotic index.
7. Preparation of Karyotype.
8. Cell Viability assay (using Evans blue stain)
9. Cell counting (using Haemocytometer).
10.Determination of blood groups.
11.Isolation of genomic DNA.
12.Detection of DNA by Gel Electrophoresis.
13.Estimation of DNA.
14.Isolation of milk protein. (casein)
15.Estimation of protein by Biuret method.
16.Assessing the enzyme acid phosphate.
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