This is not the document you are looking for? Use the search form below to find more!

Report home > Health & Fitness

Lipid Peroxidation and Antioxidant Status in Preeclampsia

0.00 (0 votes)
Document Description
The current theory suggests that in preeclampsia there is an increase in the lipid peroxidation products and leads to decrease in the serum antioxidants except uric acid, contributing to the pathogenesis of preeclampsia. In this context, this study was undertaken to determine the changes in blood levels of lipid peroxide and antioxidant levels in women with preeclampsia.Method: Case control study consisting of 30 preeclamptic and 30 healthy pregnant women. Fasting venous blood samples were collected during antepartum period and serum levels of malondialdehyde, ascorbic acid (Vitamin C) and uric acid were measured. In the preeclamptic group malondialdehyde (MDA), a lipid peroxidation product was significantly increased, while serum antioxidant ascorbic acid was significantly decreased (P< 0.001), where as uric acid level increased significantly (P<0.001). The findings of the present study are consistent with previous studies, suggesting that lipid peroxidation is an important factor in the pathogenesis of preeclampsia. In preeclampsia serum antioxidants are excessively utilized to counteract the cellular changes mediated by free radicals.
File Details
  • Added: April, 11th 2011
  • Reads: 361
  • Downloads: 4
  • File size: 61.84kb
  • Pages: 4
  • Tags: preeclampsia, lipid peroxidation, antioxidants
  • content preview
Submitter
  • Name: maurycy
Embed Code:

Add New Comment




Related Documents

Improvement effect of green tea on hepatic dysfunction, lipid peroxidation and antioxidant defence depletion induced by cadmium

by: shinta, 6 pages

We have evaluated the antioxidant effect of green tea on cadmium-induced hepatic dysfunction and stress oxidant in rats. Adult male Wistar rats were administered cadmium by injection with 20

The effects of coffee consumption on lipid peroxidation and plasma total homocysteine concentrations: a clinical trial

by: shinta, 8 pages

Despite extensive research, the cardiovascular effects of coffee consumption in humans remain controversial. Our aim was to investigate the excretion of coffee phenols and the effects of ...

Calcium and Phosphate Excretion in Preeclampsia

by: valentina, 4 pages

In preeclampsia, alterations in renal function, electrolyte and water metabolism are common findings. Recent studies have suggested that preeclampsia is associated with hypocalciuria. A total of 59 ...

THE EFFECTS OF GUAVA (PSIDIUM GUAJAVA) CONSUMPTION ON TOTAL ANTIOXIDANT AND LIPID PROFILE IN NORMAL MALE YOUTH

by: shinta, 12 pages

Fruits contain a broad spectrum of nutrients, and many of which have antioxidant properties. Phenolic substances, vitamin A, vitamin C and minerals that are present in fruits displayed ...

CURCUMIN AMELIORATES AFLATOXIN-INDUCED LIPID PEROXIDATION IN LIVER, KIDNEY AND TESTIS OF MICE : AN IN VITRO STUDY

by: shinta, 4 pages

The present investigation was an attempt to evaluate the possible ameliorative effect of curcumin on aflatoxin-induced lipid peroxidation in liver, kidney and testis of mice in vitro. Tissues ...

Spinacia oleracea L. protects against gamma radiations : a study on glutathione and lipid peroxidation in mouse liver

by: shinta, 9 pages

The present study deals with the protective effect of Spinacia oleracea L. against radiation-induced oxidative stress, which is evaluated in terms of lipid peroxidation (LPO) product and tissue ...

Leptin levels and antihypertensive treatment in preeclampsia

by: clara, 5 pages

This study was carried out to investigate changes in the plasma leptin concentrations during preeclampsia treatment and to determine whether antihypertensive treatments, aimed at decreasing leptin ...

The correlation between plasma homocysteine and malondialdehyde levels in preeclampsia

by: michael, 4 pages

The aim of this paper is to investigate the possible correlation between plasma malondial-dehyde and homocysteine levels of preeclamptic patients.

Differing views on women's status in a protestant society 1

by: 481168, 1 pages

differing views on women's status in a protestant society part 1

FPSOs will Play a Key Role in Pre-salt Oil and Gas Projects in Brazil

by: charlesmartin17, 2 pages

Offshore projects contribute over 90% to Brazil’s oil and gas production of about 860 million barrels of oil equivalent (MMboe), owing to the rapid development of deep water and ultra deep ...

Content Preview
O R I G I N A L A R T I C L E





A l A m e e n J M e d S c i ( 2 0 1 0 ) 3 ( 1 ) : 3 8 - 4 1
I S S N 0 9 7 4 - 1 1 4 3

Lipid Peroxidation and Antioxidant Status in Preeclampsia



S.V. Kashinakunti 1*, Sunitha H1, K. Gurupadappa 1, D.S.
Shankarprasad 1, G.Suryaprakash 2 and J.B Ingin 2

1Department of Biochemistry. S. Nijalingappa Medical College, H.S.K. Hospital
Research Centre Bagalkot, Karnataka, India and 2Department of Biochemistry, M.R.
Medical College, Gulbarga, Karnataka, India.

Abstract
The current theory suggests that in preeclampsia there is an increase in the lipid peroxidation
products and leads to decrease in the serum antioxidants except uric acid, contributing to the
pathogenesis of preeclampsia. In this context, this study was undertaken to determine the
changes in blood levels of lipid peroxide and antioxidant levels in women with
preeclampsia.Method: Case control study consisting of 30 preeclamptic and 30 healthy
pregnant women. Fasting venous blood samples were collected during antepartum period and
serum levels of malondialdehyde, ascorbic acid (Vitamin C) and uric acid were measured. In
the preeclamptic group malondialdehyde (MDA), a lipid peroxidation product was
significantly increased, while serum antioxidant ascorbic acid was significantly decreased (P<
0.001), where as uric acid level increased significantly (P<0.001). The findings of the present
study are consistent with previous studies, suggesting that lipid peroxidation is an important
factor in the pathogenesis of preeclampsia. In preeclampsia serum antioxidants are
excessively utilized to counteract the cellular changes mediated by free radicals.
Keywords. : Preeclampsia, Lipid peroxidation, Antioxidants

Introduction
Pregnancy is a physiological stress in which many changes occur in the milieu
interior of the body, more and more stress is being laid on the biochemical changes,
which occur in the blood during normal pregnancy becomes exaggerated in
complications of pregnancy like pre-eclampsia. Pre-eclampsia occurs during second
and third trimester of pregnancy and it is more common in nulliparous women. It is
characterized by 1) BP 140/90 mm Hg or greater at least on two occasions, six or
more hours apart 2) Proteinuria ≥300 mg/24 hours or greater or equal to 1+ by
dipstick response[1]. Oxidative stress increases during pre-eclampsia and results in
increased production of lipid peroxides, reactive oxygen species and superoxide
anion radicals to cause endothelial injury and dysfunction, platelet and neutrophil
activation[2-3]. These observations in pre-eclampsia have given rise to increased
interest in antioxidants such as Ascorbic acid(Vitamin C), Vitamin E and Uric acid.
In this context the present study has been undertaken to determine the changes in
serum levels of peroxidation product ie Malondialdehyde (MDA) and antioxidant
levels namely ascorbic acid and uric acid in women with pre-eclampsia.
Materials and Methods
In the present study 30 cases of pre-eclampsia and 30 normal pregnant women from
HSK (Hangal Shree Kumareshwar) Hospital and Research Centre Bagalkot were
selected. The controls were normotensives. Both the cases and controls were primi



© 2010.Al Ameen Charitable Fund Trust, Bangalore
38

Al Ameen J Med. Sci, Volume3, No.1, 2010 Kashinakunti SV .et al

between 18 and 30 years of age and having more than 29 weeks of gestation. The
cases and controls having past history of diabetes mellitus, hypertension, renal
diseases, liver disorder and with the history of multivitamin intake were excluded.
History and examination findings of both cases and control were noted. Fasting
Blood samples were collected, the serum was separated and analyzed for the
following parameters. MDA was measured by Thiobarbituric acid reactive
substances assay (TBRAS) method[4] and ascorbic acid by Dinitrophenyl
Hydrazine ( DNPH) method.[5] The uric acid was measured by uricase method using
Human diagnostics kit[6-8]. The optical densities of MDA and ascorbic acid were
measured at 532 nm and 520nm respectively using spectrophotometer.

Results
Blood pressure and BMI (Body Mass Index) of cases and controls is shown in Table
1. The mean value of SBP (Systolic blood pressure) in cases is 144.67±10.56 and in
control 115.3±6.81,there is significant difference (P<0.001) between cases and
controls. The DBP(Diastolic Blood Pressure) mean in cases and control is 97.73±5.7
and 75.45 ± 5.25 respectively, there is significant difference (P<0.001) between
cases and controls. The mean BMI ( Body Mass Index) of controls is 25.53± 3.08
and in study group 26.83± 3.28, P value is more than 0.05 , which is insignificant.
Table-1: Blood Pressure and Body Mass Index of Cases and Controls


Cases = 30
Controls n = 30
P Value
BP
SBP mm Hg 144.67 ± 10.56
115.3 ± 6.81
P < 0. 00 1

DBP mm Hg 97.73 ± 5. 7
75.45 ± 5.25
P < 0. 00 1
BMI (kg/M2)
26.83 ± 3.28
25.53 ± 3.08
P > 0.05
Values are mean ±SD; BP :Blood Pressure , SBP, Systolic blood pressure; DBP, Diastolic blood
pressure ;BMI, Body mass index

Biochemical parameters to assess lipid peroxidation and antioxidant status of cases
and controls were illustrated in Table-2.

Table- 2:Biochemical parameters to assess lipid peroxidation and antioxidant status of cases
and controls
Biochemical
Cases n = 30
Controls n = 30
P Value
Parameter
MDA n mol/L
346.13 ± 110.54
205.07 ± 26.06
P < 0. 001
S. Ascorbic acid mg/dl
0.62 ± 0.21
0.97 ± 0.19
P < 0. 001
S. Uric acid mg/dl
7.16 ± 1.68
4.75 ± 0.91
P < 0. 001
Values are mean ±SD; MDA : Malondialdehyde.




© 2010. Al Ameen Charitable Fund Trust, Bangalore
39

Al Ameen J Med. Sci, Volume3, No.1, 2010 Kashinakunti SV .et al

The mean MDA in cases and controls is 346.13±110.54 nmol/L and 205.07±26.06
nmol/L respectively. MDA is significantly more in pre-eclampsia patients compared
to controls ( P<0.001). The mean ascorbic acid level in cases and control is 0.62 ±
0.21 mg/ dl and 0.97 ± 0.19 mg/dl respectively. There is significant decrease in
ascorbic acid levels (P<0.001) in cases as compared to controls. The mean uric acid
level in cases and controls is 7.16±1.68mg/dl and 4.75±0.91 mg/dl, respectively.
There is significant elevation of uric acid (P<0.001) in study group in comparison to
controls.
Discussion

The pathophysiology of pre eclampsia is poorly understood. Free radicals and other
damaging reactive oxygen species, such as the superoxide anions are in oxidative
metabolic processes, their activation is thought to increase during pre-eclampsia[9].
Foetoplacental unit may be the origin of oxygen free radicals and lipid peroxides[10-
11].Recent investigation suggested that endothelial cell injury may be the initiator of
the pathophysiological events of pre-eclampsia[12]. Either placental hypercorrection
of lipid peroxides or decreased placental antioxidant enzyme activity can lead to
endothelial dysfunction. Insufficient antioxidant capacity leads to oxidative stress
and subsequently oxidative injury may occur in both the maternal and placental
compartments9. A number of reports indicate that blood levels of lipid peroxidation
products are elevated in women with pre-eclampsia relative to normal pregnancy
[3,9,13-15]. Furthermore placental production of lipid peroxides have been
demonstrated to be abnormally increased in pre-eclampsia10, consistent with previous
reports, in the present study also there is increase in serum levels(P<0.001) of MDA
in the pre-eclamptic patients. Pre-eclampsia is associated with increased utilization of
antioxidants. Several studies have demonstrated decreased serum levels of ascorbic
acid compared to normal pregnant women, [3,11,16] similarly in the present study
we have observed a significant decrease in serum levels of ascorbic acid in the pre-
eclamptic patients(P<0.001). Uric acid is water soluble and a weak serum
antioxidant. Nevertheless, the patients with pre-eclampsia show hyperuricemia,
which mean that the serum levels doesn’t protect the pre-eclamptic patients against
free radical activity [9,16-17]. The present study has also shown a significant rise in
serum uric acid (P<0.001). The rise in uric acid in pre-eclampsia is not merely a non-
specific reflection of kidney damage, but a sign of antioxidative response, possibly
related to the pathogenesis of pre-eclampsia[13]. In conclusion the present study is
consistent with previous studies suggests that lipid peroxidation appears to be of
immense value in understanding the pathogenesis of preeclampsia.In preeclamptic
patients antioxidants may be utilized to a greater extent to counteract free radical
mediated cellular changes, resulting in the reduction of plasma antioxidant levels.

References
1.
Cummingham FG, Grant NF, Leveno KJ, Gilstrap LCIII, Hanth JC, Wenston KC in
“Hypertensive disorders in pregnancy”, Williams Obstetrics, 21st Edition, McGraw Hill
pp 568-569,572.
2.
Dutta DC, Konar HL, Test Book of Obstetrics, 6th edn. New Central Book Agency
( P) Ltd. 2004 ,pp222-223.

© 2010. Al Ameen Charitable Fund Trust, Bangalore
40

Al Ameen J Med. Sci, Volume3, No.1, 2010 Kashinakunti SV .et al


3.
Riza Madazli,Ali Benian, Koray Gumata et al. Lipid peroxidation and antioxidants in
pre Eclampsia. Eur J Obstet Gynecol & Reprod Biol 1999; 85 (2): 205-208.
4.
Draper HH, Hadley M. Methods of Enzymol. 1990-, pp 421-431.
5.
Weinstein L.Syndrome of haemolysis, elevated liver enzymes and the platelet count. A
severe consequence of hypertension in pregnancy. Am J Obstet Gynecol 1982; 142 :
159-167.
6.
Barham D, Trinder P. An improved colour reagent for the determination of blood
glucose by the oxidase system. Analyst 1972; 97 : 142-145
7.
Fossati LP, Berti G. Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-
aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and
urine. Clin Chem 1980;26(2):227-231.
8.
Thefeld L. Dtsch. Untersuchungen zur Frage der Hämodialyse bei der
Knollenblätterpilzvergiftung.Med. Wschr 1973; 98: 300.
9.
Mehmet Harma, Muge Harma and Ozcan Erel : Measurement of total antioxidant
response in pre-eclampsia with a novel automated method. Eur J Obstet Gynecol &
Reprod Biol
2005;118 (1) : 47-51.
10. Poranen AK, Ekblad U, Uotila P, Ahotupa M. Lipid peroxidation and antioxidants in
normal and pre-eclamptic pregnancies. Placentas 1996; 17: 401-405.
11. Wang Yand ,Walsh SW. Antioxidant activities and mRNA expression of superoxide
dismutase, catalase and glutathione peroxidase in normal and pre-eclamptic placenta. J
Soc Gynecol Investig
1996; 3 : 179-184
12. Simmi Kharb, Sharina BD. Vitamin E and C in preeclampsia. Euro J Obstet. & Gyneco.
Reprod Biol 2000; 93 (1) : 37-39.
13. Utoila JT, Tuimala RJ, Aarnio TM, Pyykko KA, Ahotupa MO, Findings on lipid
peroxidation and antioxidant function in hypertensive complications of pregnancy. Br J
Obstet Gynecol
1993:100: 270-276.
14. Jain SK , Wise R, Relationship between elevated lipid peroxides, vitamin E deficiency
and hypertension in pre-eclampsia. Mol Cell Biochem 1995 : 151, 33-38.
15. Kaur Gurjit, Mishra S, Sehgal A, Prasad R. Alterations in lipid peroxidation and
antioxidant status in pregnancy with prececlampsia. Mol Cell Biochem 2008, 313(1-
2):37-44.
16. Chappell LC, Seed PT., Briley A, Kelly SRN, Frank J, Hunt BJ et al. A longitudinal
study of biochemical variables in women at risk of preeclampsia. Am J Obstet Gynecol
2002, 187(1):127-136.
17. Magann, EF, Martin JNJr. The Laboratory Evaluation of Hypertensive Gravidas.
1995, 50(2): 138-145

*All correspondences to: Kashinakunti.SV.,Assistant Professor, Department of Biochemistry, S.Nijalingappa
Medical College,Navanagar, Bagalkot – 587102, Karnataka,India.Email : drsvkashinakunti@yahoo.co.in

© 2010. Al Ameen Charitable Fund Trust, Bangalore
41

Download
Lipid Peroxidation and Antioxidant Status in Preeclampsia

 

 

Your download will begin in a moment.
If it doesn't, click here to try again.

Share Lipid Peroxidation and Antioxidant Status in Preeclampsia to:

Insert your wordpress URL:

example:

http://myblog.wordpress.com/
or
http://myblog.com/

Share Lipid Peroxidation and Antioxidant Status in Preeclampsia as:

From:

To:

Share Lipid Peroxidation and Antioxidant Status in Preeclampsia.

Enter two words as shown below. If you cannot read the words, click the refresh icon.

loading

Share Lipid Peroxidation and Antioxidant Status in Preeclampsia as:

Copy html code above and paste to your web page.

loading