Study on Grape Seeds Extraction and Optimization : An Approach

The efficient methods for extracting phenolics from grape seeds have shown broad range of pharmacological activities, due to the special health promoting and disease preventing effects of polyphenols. In this research, red grape seeds were subjected to different extraction conditions. The effect of single factor such as the concentration of solvent, the ratio of liquid to solid (L/S), extraction temperature and extraction time were studied. Total phenol was measured by the 4-ammonium antipyrine method to monitor the efficiency of the extraction under different experimental conditions. High Pressure Liquid Chromatograph (HPLC) analysis was applied to identify and quantify some specific dimeric and trimeric phenolics in grape seeds. The extract with highest total phenolics was obtained with ethanol when the ratio of ethanol to water was 1:1 to 3:2 (v/v). A higher extraction yield was obtained at higher temperature. In most cases, when ratio of liquid to solid was 7-8:1 and extraction time was around 3 h resulted in the best yield.