The role of process analytical technology (pat) in green chemistry and green engineering webinar

The Role of Process Analytical Technology (PAT) in Green Chemistry and Green Engineering – Part IITuesday December 1st4am, 9am, and 2pm ESTPresenter: Dominique Hebrault, Ph.D.Senior Technology and Application ConsultantThe Twelve Principles of Green Chemistry1Green Chemistry and Continuous or Bio ProcessGreen Chemistry and Continuous or Bio ProcessGreen Chemistry and Continuous or Bio ProcessOutline Case Studies- Monitoring of a Biotransformation using ReactIR™- Development of a Continuous Process with ReactIR™- RC1e Calorimetry: a Tool for Continuous Process Development- Bioprocess Monitoring using RC1e Calorimetry Conclusion5Case Study: FTIR as PAT tool for BiotransformationMonitoring of Baeyer-Villiger bio-transformation kinetics and finger-printing using ReactIR spectroscopy IntroductionMost fermentation monitoring concernsthedeterminationofanalyteconcentrationsReactIR™ used for:- Measuring progress and kinetics- Conversion of cyclododecanone (CDD) into lauryl lactone (LL)- Catalyzed by a recombinant NADPH-dependent cyclopentadecanone monooxygenaseSource: Peter C.K. Lau et al, Biotechnology Research Institute, National Research Council, Canada; Industrial Biotechnology 2006, 138–142; Applied and Environmental Microbiology, 2006, 2707–2720Case Study: FTIR as PAT tool for BiotransformationResults of CDD biotransformation as a function of cell growth in a fed-batch cultureQualitative: 3-D spectral fingerprint of CDD conversion to LL shows:- Decrease of CDD absorbance at 1713cm-1- Increase of LL absorbance at 1741cm-1Quantitative: Peak profiling and quantitative calibration model using QuantIRTM to monitor- Use of authentic standards of CDD and LL- Detection sensitivity for LL: 0.2 mMSource: Peter C.K. Lau et al, Biotechnology Research Institute, National Research Council, Canada; Industrial Biotechnology 2006, 138–142; Applied and Environmental Microbiology, 2006, 2707–2720Case Study: FTIR as PAT tool for Biotransformation-Better understanding of reaction kinetics-Original utilization of ReactIR™technology for offline qualitative and quantitative monitoring of cyclododecanone biotransformation-Further development in online monitoring and automatic controlling-Initial expansion to a wider range of cycloketonesSource: Peter C.K. Lau et al, Biotechnology Research Institute, National Research Council, Canada; Industrial Biotechnology 2006, 138–142; Applied and Environmental Microbiology, 2006, 2707–2720Outline Case Studies- Monitoring of a Biotransformation using ReactIR™- Development of a Continuous Process with ReactIR™- RC1e Calorimetry: a Tool for Continuous Process Development- Bioprocess Monitoring using RC1e Calorimetry Conclusion9